In several circumstances, baseline separation on the peaks is usually attained only with gradient elution and small column loadings. Hence, two drawbacks to elution method chromatography, Particularly at the preparative scale, are operational complexity, as a consequence of gradient solvent pumping, and minimal throughput, resulting from very low c
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Incorporate a recognized amount of the antidepressant protriptyline, which serves being an inside regular, to every serum sample and to each external conventional. To get rid of matrix interferents, pass a 0.5-mL aliquot of each serum sample or conventional by way of a C18 stable-phase extraction cartridge. Just after washing the cartridge to remov
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